Basic study of the peroxide production in mouse's tissue by X-ray irradiation, and protective effects of the anti-oxidizing agent(extracts from basidiomycetes) on radiation damages.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461906
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Radiation science
• Research Institution: Chiba University
• Principal Investigator: UNO TAKASHI 千葉大学, 医学(系)研究科(研究院), 教授 (30302540)
• Co-Investigator(Kenkyū-buntansha): HORIKOSHI TAKURO 千葉大学, 医学部附属病院, 助教 (50456068) ; HARADA RINTARO 千葉大学, 医学部附属病院, 助教 (20568662) ; SAITO MASAYOSHI 千葉大学, 大学院医学研究院, 特任研究員 (80118885) ; INOUE KOUHEI 千葉大学, 医学部附属病院, 助教 (40312053) ; ISOBE KOUICHI 東邦大学, 医学部, 准教授 (80334184)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 放射線治療生物学 / 過酸化物 / 放射線防護

Outline of Final Research Achievements

This present study was planned to investigate on peroxide productions (8-OHdG and 8-BrdG) after the X-ray exposure to mice, and protection effects from radiation damages by antioxidant prescription (extracts from basidiomycota : ”extracts”).
(1) The peroxide of DNA (8-OHdG) was detected in 4.5Gy irradiated mice plasma for 5 to 7 days after irradiation. The positive spots (8-OHdG) of thymus and spleen preparations in mice were detected in 5 days after irradiation. The positive spots of an oxidative stress marker (8-BrdG) in thymus, spleen were detected in 1 day and/or 3days after irradiation. (2) The survival rate of the male ICR mice exposed to 8.0Gy whole-body X-ray irradiation, all administered groups of “extracts” were increase the survival rate. The radiation death was caused by the radiation intestinal damages (sepsis by mouse’s enteric bacteria). The sperm count and lymphocyte activity in irradiated mice were suggested to elevated by “extracts” administration.

Free Research Field

医学薬学