Metabolome analysis of invaded pancreatic cancer cell line, PANC-1, after irradiation

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461934
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Radiation science
• Research Institution: National Institute of Radiological Sciences
• Principal Investigator: Imai Takashi 国立研究開発法人放射線医学総合研究所, 重粒子医科学センター, プログラムリーダー (50183009)
• Co-Investigator(Renkei-kenkyūsha): Ishikawa Atsuko 国立研究開発法人放射線医学総合研究所, 重粒子医科学センター, 主任技術員 (30443063)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 転移 / 浸潤 / 粒子線治療 / メタボローム解析 / 代謝経路

Outline of Final Research Achievements

The aim of this study is to identify the metabolic pathways characterized in the invaded cancer cells, PANC-1, whose invasiveness is enhanced after the carbon-ion (C-ion) irradiation, and propose the new candidates reducing the C-ion enhanced invasiveness. Metabolome analysis using CF-TOFMS showed that several metabolites in the glycolytic pathway, the uric acid pathway synthesizing arginine, and the folate pathway producing glutathione were all increased in the invaded PANC-1 cells compared to those of whole-cultured PANC-1 cells. Such increases in the metabolite were also shown in the invaded cells after the C-ion irradiation. From the results, we next clarified the enzymes responsible for the synthesis of increased metabolites found in the invaded cells. This approach would be useful for identifying new inhibitors for the invasion of cancer cells.

Free Research Field

放射線生物学