2015 Fiscal Year Final Research Report
Radiation sensitivity in dormant tumor cells using a novel primary culture system
| Project/Area Number |
25461937
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Research Institute, Osaka Medical Center for Cancer and Cardiovascular Disaeses |
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Principal Investigator |
Endo Hiroko 地方独立行政法人大阪府立病院機構大阪府立成人病センター(研究所), 研究所, 研究員 (20359300)
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| Co-Investigator(Kenkyū-buntansha) |
Inoue Masahiro 地方独立行政法人 大阪府立病院機構大阪府立成人病センター(研究所), 研究所, 部長 (10342990)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 大腸癌 / 放射線感受性 / 休眠状態 |
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| Outline of Final Research Achievements |
CTOS (cancer tissue-originated spheroid) from colorectal cancer patients entered a reversible dormant status when cultured in hypoxia and growth factor deprived conditions. CTOS retained the characteristics of highly differentiated adenocarcinoma, and the expression of stemness or differentiation genes changed drastically depending on the culture conditions.
In the present study, we established a radiation sensitivity assay using colon cancer CTOS. CTOS in dormant status showed radiation resistance compared to the normal culture conditions, and dormant CTOS showed high expression of stemness and differentiated genes at the same time. In addition to the well-known radio-resistant mechanism, such as cell cycle arrest and hypoxia, dormant CTOS might gain the radiation resistance through the fluctuation of stemness and differentiation.
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| Free Research Field |
医歯薬学
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