The role of Sonic hedgehog in intestinal injury via stem cell

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461951
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General surgery
• Research Institution: The University of Tokushima
• Principal Investigator: YOSHIKAWA Kozo 徳島大学, 大学病院, 助教 (80448331)
• Co-Investigator(Kenkyū-buntansha): BANDO Yoshimi 徳島大学, 病院, 准教授 (00238239) ; KURITA Nobuhiro 徳島大学, 病院, 特任教授 (30335814) ; SATO Hirohiko 徳島大学, 病院, 特任助教 (80609694)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 腸管修復 / Sonic hedgehog / 幹細胞

Outline of Final Research Achievements

This study focused on the relationship between the intestinal injury and Sonic hedgehog pathway. In intestinal injury models, Shh pathway was downregulated. HCT-116 sphere-derived cells showed significantly increased expression of stem cell markers, Shh downstream genes and epithelial-mesenchymal transition (EMT) markers compared to parental cells grown in conventional culture. The expression of stemness markers, Shh downstream genes and EMT markers were higher in cancer spheres than the parental cell line and downregulated by cyclopamine treatment in a dose-dependent manner. Shh pathway is related with cancer stem cell and may have an important role in intestinal injury.

Free Research Field

消化器外科