Novel spatiotemporal analysis of mechanism for rejection after liver transplantation using two-photon microscopy

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461954
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General surgery
• Research Institution: Kumamoto University
• Principal Investigator: takeichi takayuki 熊本大学, 医学部附属病院, 非常勤診療医師 (00380999)
• Co-Investigator(Kenkyū-buntansha): INOMATA Yukihiro 熊本大学, 大学院生命科学研究部, 教授 (50193628) ; HONDA Masaki 熊本大学, 大学院生命科学研究部, 助教 (80573609)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: マウス同所性肝移植 / ラット同所性肝移植 / 肝虚血再灌流障害 / 好中球動態 / 2光子励起顕微鏡 / 急性拒絶反応

Outline of Final Research Achievements

We showed a novel method for examining neutrophil recruitment in liver transplant model and hepatic ischemia-reperfusion(I/R) injury model using two-photon microscopy.
In liver transplant model, we could observe the blood circulation in sinusoids and the behavior of neutrophils.
In I/R model, at low magnification, four to six hepatic lobules could be visualized. The number of adherent neutrophils continued to increase for 4 hr after reperfusion, whereas their crawling velocity reached a maximum of 2 hr after reperfusion and then decreased gradually. High-magnification images revealed the presence or absence of blood circulation in sinusoids. Adherent neutrophils in perfused areas in the I/R group had more elongated shapes and moved more quickly than those in nonperfused areas and in the control group.

Free Research Field

医歯薬学