Comprehensive analysis of expression and function of lincRNA in gastroenterological cancer

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461984
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General surgery
• Research Institution: Japanese Foundation for Cancer Research (2015); Kumamoto University (2014); Kyushu University (2013)
• Principal Investigator: Hiyoshi Yukiharu 公益財団法人がん研究会, その他部局等, その他 (30573612)
• Co-Investigator(Kenkyū-buntansha): WATANABE Masayuki がん研有明病院, 消化器外科 (80254639)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 消化器癌 / non-coding RNA / real-time PCR / 組織 / 血清

Outline of Final Research Achievements

The expression levels of lncRNA (HOTAIR, Malat1, MEG3 and GAS5) were analyzed in cancer tissue and adjacent normal epithelium of patients with gastrointestinal cancer by real-time PCR. HOTAIR was up-regulated in cancer tissue compared with adjacent normal epithelium in patient with esophageal cancer, gastric cancer and colorectal cancer. However, the expression levels of Malat1, MEG3, GAS5 were not significantly changed between cancer tissue and normal epithelium. There was no significant correlation between the expression of these lncRNAs and patient survival. Then, we tried to analyze the expression of lncRNA in serum of patient with colorectal cacner. However, we could not quantify the expression because the expression levels was too low.
In summary, we could not identify specific lncRNAs as hopeful biomarker or therapeutic target in gastrointestinal cancer.

Free Research Field

消化器癌の外科治療