2015 Fiscal Year Final Research Report
The Novel Comprehensive Screening of Anticancer Drug Resistant Genes of Breast Cancer Cell
| Project/Area Number |
25461997
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
General surgery
|
|---|
| Research Institution | Keio University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
Hayashida Tetsu 慶應義塾大学, 医学部, 講師 (80327543)
Jinno Hiromitsu 帝京大学, 医学部, 教授 (20216261)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Keywords | 薬剤耐性 / スクリーニング / トランスポゾン / 乳癌 |
|---|
| Outline of Final Research Achievements |
Drug resistance is one of the major issues in managing esophageal cancer patients as chemotherapy is a pillar treatment today. Since drug resistance may be mediated by genetic changes in the tumor, the identification of gene mutation may lead to better outcomes in therapy. We performed a novel method using transposon for a comprehensive screening with a view to identify drug resistant genes. Transposons are DNA sequences that move from one location on the genome to another. By this nature, a modified piggyBac transposon was designed as an insertion mutagen. A cytomegalovirus (CMV) promoter sequence was added to initiate strong transcription. When the transposon is inserted to the upstream of a certain gene, the gene will be up-regulated while when inserted downstream of intragenicaly it will be down regulated. The method is inexpensive and relatively simple, and capable both in activation and disruption, leading to comprehensive screening.
|
| Free Research Field |
乳腺外科学
|
