Differential expression of type IV collagen alpha3 (IV), alpha4 (IV), and alpha5 (IV) chains in non-medullary thyroid carcinoma.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462001
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General surgery
• Research Institution: Aichi Medical University
• Principal Investigator: Kousaka Junko 愛知医科大学, 医学部, 助教 (50440748)
• Co-Investigator(Kenkyū-buntansha): NAKANO Shogo 愛知医科大学, 乳腺・内分泌外科, 教授 (20351108) ; FUJII Kimihito 愛知医科大学, 乳腺・内分泌外科, 准教授 (00524331) ; SHIOMI Yukako (MOURI Yukako) 愛知医科大学, 乳腺・内分泌外科, 助教 (90465576) ; ANDO Takahito 愛知医科大学, 乳腺・内分泌外科, 助教 (40634134)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: Type IV collagen / Basement membrane / Second network / Thyroid cancer / Non medullary cancer / Differential potential

Outline of Final Research Achievements

The majority of thyroid cancers except medullary carcinoma are derived from thyroid follicular epithelial cells. To evaluate the distribution of second BM network of type IV collagen consisted of alpha 3, 4, 5(IV) chains in non-medullary thyroid carcinoma, we investigated the cellular regulation of these alpha (IV) chains in 70 well differentiated thyroid carcinomas (WDTC) (58 papillary, 12 follicular), and eight poorly differentiated and undifferentiated thyroid carcinomas (PUDTC) (three poorly, five undifferentiated) by immunohistochemistry by using alpha (IV) chain-specific antibodies. In normal thyroid tissues, BMs of thyroid gland were composed of discontinuous alpha 3, 4, 5(IV) chains. Similar profiles were observed in 61 cases (88%) of WDTC. On the other hands, in 6 cases (75%) of PUDTC, the assembly of alpha 3, 4, 5(IV) chains was completely inhibited. The expression of second BM network seems to be associated with the differential potential of non-medullary thyroid carcinoma.

Free Research Field

内分泌外科学