2015 Fiscal Year Final Research Report
Development of safer and more useful materials for regenerative medicine mimicking the properties of amniotic membrane
| Project/Area Number |
25462002
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Doshisha University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HAGIWARA AKEO 同志社大学, 生命医科学部, 教授 (90198648)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 再生医学 / 羊膜 / )再生医療材料 / 実験外科学 |
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| Outline of Final Research Achievements |
We performed the basic study to create more safe and functional materials, which are mimicking amniotic membrane as an excellent material for regenerative medicine. In this study, we investigated the effects of anti-adhesion and peritoneal regeneration for the membrane made of alkali- or acid-treated gelatin or their complexes, containing the culture medium of stem cells established from amniotic membrane (conditioned medium: CM). The culture studies showed that CM promoted the growth of mesothelial cells and that it was more effective to condense CM at 5-10 times. From the studies using rat cecum adhesion model, the freeze dry membrane made of alkali-treated gelatin with condensed CM had most excellent anti-adhesive effect and peritoneal regeneration.
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| Free Research Field |
医歯薬学
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