2015 Fiscal Year Final Research Report
Molecular mechanism of solid tumor growth inhibition by a new differentiation inducer and molecular target agents
| Project/Area Number |
25462007
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
General surgery
|
|---|
| Research Institution | Shimane University (2014-2015)
Research Institute for Clinical Oncology, Saitama Cancer Center (2013) |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Keywords | コチレニンＡ / 三酸化ヒ素 / 分化誘導剤 / 乳癌細胞 / 増殖抑制 / 併用効果 / アポトーシス / 活性化酸素 |
|---|
| Outline of Final Research Achievements |
We have examined whether a new differentiation inducer of leukemia cells can suppress the proliferation of solid tumor cells. In this study we found that cotylenin A significantly potentiate arsenic trioxide-induced inhibition of cell growth of human breast and pancreatic cancer cells. The combined treatment with cotylenin A and arsenic trioxide induced cleaved caspase-7 in human breast cancer MCF-7 cells at the concentration which arsenic trioxide alone scarcely induced and cotylenin A alone only weakly induced. Expression of survivin was markedly decreased with the presence of both cotylenin A and arsenic trioxide. The pretreatment with N-acetylcysteine significantly reduced the combination treatment-induced cell growth inhibition. These data suggest that induction of cleaved caspase-7, inhibition of survivin and oxidative responses are important events in the corporative inhibition in the growth of MCF-7 cells.
|
| Free Research Field |
医歯薬学
|
