A study for immunological feature of tumor endothelial cells (TEC) in immune evasion mechanism of tumor micro-environment and development of a novel anti-cancer therapy

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462008
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General surgery
• Research Institution: Department of Clinical Research, National Hospital Organization Kure Medical Center
• Principal Investigator: Onoe Takashi 独立行政法人国立病院機構(呉医療センター臨床研究部), その他部局等, その他 (90549809)
• Co-Investigator(Renkei-kenkyūsha): OHDAN HIDEKI 国立大学法人 広島大学, 大学院医歯薬保健学研究院 消化器・移植外科学, 教授 (10363061)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 腫瘍免疫 / 免疫逃避 / 内皮細胞 / 微小環境 / クロストーク

Outline of Final Research Achievements

Although a remarkable progress in anti-cancer therapies, caner can be resistant to therapies in some situations, which could related to immune-evasion of cancer. In this study, we evaluated an immunological feature of tumor endothelial cells (TEC) in immune evasion mechanism of tumor micro-environment. In vitro assay revealed that TECs from growing tumor in mice in which B16 melanoma cells had been implanted suppressed T cell proliferation in a dose-dependent manner. Further, tumor-endothelial cell serial culture assay revealed that immunosuppressive function of TEC required HMGB1 from tumor and exosome secreted from TEC had immunosuppressive activity. In vivo animal model, tumor growth was suppressed by local injection of anti-HMGB1 antibody. These results suggest that TECs contribute immune-evasion of tumor by acquiring immunosuppressive feature through cross-talk with tumor and blockade of this cross-talk could be a promising anti-cancer strategy.

Free Research Field

外科学