2015 Fiscal Year Final Research Report
Investigation of an EMAST-generating tumor microenvironment and its relating malignant progression for colorectal cancer
| Project/Area Number |
25462071
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Digestive surgery
|
|---|
| Research Institution | Toho University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
FUNAHASHI Kiminiko 東邦大学, 医学部, 教授 (90297698)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Keywords | 癌微小環境 / 進行性大腸癌 / 低酸素 / 転移 / ゲノム不安定性 / EMAST |
|---|
| Outline of Final Research Achievements |
The molecular mechanisms of progression and metastasis must be elucidated to cure metastatic colorectal cancers (CRCs). In this study, we established a new culture condition of colorectal cancer cell lines to generate elevated microsatellite alterations at selected tetra-nucleotide (EMAST). EMAST is a type of genomic instability and frequently detected in poor prognosis CRCs. Therefore, the in vitro EMAST generating condition is thought to reflect in vivo tumor microenvironment for malignant progression. Under this condition, we identified several genes with no apparent relevance to CRC by a comprehensive analysis of genes expression. Caveolin-1 (CAV1) was a candidate for novel malignant CRC-relating protein, because it was also detected in clinical CRC samples. Although precise roles of identified genes, including CAV1, were not reveled, our established EMAST-gerating culture condition was shown to be useful for a screening of malignant CRC-relating factors.
|
| Free Research Field |
腫瘍分子生物学
|
