2016 Fiscal Year Final Research Report
Promoting liver regeneration using an ATP-sensitive K+ channel opener
| Project/Area Number |
25462077
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Akita University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | K-ATPチャネル / 肝細胞 / 残肝増殖能 / Diazoxide / ミトコンドリア / マイクロアレイ / カルシウムチャネル |
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| Outline of Final Research Achievements |
We previously reported that the ATP-sensitive potassium (K-ATP) channel existed in mitochondria of rat hepatocyte, and K-ATP channel opener (diazoxide: Dz) promoted regeneration of remnant liver after hepatectomy. Furthermore, we showed that diazoxide decreased Ca ion release from intracellular Ca ion storing organellae in isolated hepatocytes. To solve the reason why K-ATP channel opener enhances liver regeneration, we analysed gene expression cyclopaedically. As a result, mRNAs of RyR2 and calmodulin decreased in Dz group with a significant difference. In related genes to apoptosis, the expression of caspase-3 and BAX changed. DNA microarray analysis showed the upregulations of genes related to cell adhesion and neural factor.
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| Free Research Field |
肝胆膵外科学
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