Development of Adipose derived meschymal stem cell therapy for liver cirrhosis

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462096
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kyushu University
• Principal Investigator: Akahoshi Tomohiko 九州大学, 医学(系)研究科(研究院), 准教授 (20336019)
• Co-Investigator(Kenkyū-buntansha): Hashizume Makoto 九州大学, 大学院医学研究院, 教授 (90198664) ; Murata Masaharu 九州大学, 大学院医学研究院, 准教授 (30304744) ; 富川 盛雅 九州大学, 大学院医学研究院, 特任准教授 (60325454)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 間葉系幹細胞 / 肝硬変 / 幹細胞治療 / 門脈圧亢進症

Outline of Final Research Achievements

Background:Recent studies show that adipose tissue-derived mesenchymal stem cells(ADSC) have potential clinical applications. Here, we investigated the effect of adipose tissue-derived mesenchymal stem cells infusion on a liver fibrosis rat model and elucidated the underlying mechanism.Result:bFGF treatment markedly improved the proliferation, differentiation, and hepatocyte growth factor expression ability of ADSC. Although ADSC infusion alone slightly ameliorated liver functions and suppressed fibrosis progression, bFGF-treatment significantly enhanced the therapeutic effect in association with elevated hepatocyte growth factor expression. Co-culture with ADSC led to induction of hepatic stellate cell apoptosis and enhanced hepatocyte proliferation. Mechanism investigation revealed that co-culture with adipose tissue-derived mesenchymal stem cells activated c-jun N-terminal kinase-p53 signaling in hepatic stellate cell and promoted apoptosis.

Free Research Field

消化器外科