Construction of liver tissue in vivo with preparative partial hepatic irradiation and growth stimulus: investigations of less invasive techniques and progenitor cells.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462097
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Nagasaki University
• Principal Investigator: YAMANOUCHI Kosho 長崎大学, 医歯薬学総合研究科(医学系), 講師 (10448508)
• Co-Investigator(Kenkyū-buntansha): EGUCHI Susumu 長崎大学, 医歯薬学総合研究科(医学系), 教授 (80404218) ; SAKAI Yusuke 長崎大学, 医歯薬学総合研究科(医学系), 助教 (10608904)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 再生医療 / 脂肪組織由来多能性幹細胞 / 肝放射線照射 / 増殖刺激

Outline of Final Research Achievements

The selective proliferation of transplanted hepatocytes with a growth stimulus, such as portal vein branch ligation (PVBL), concomitant with hepatic irradiation (HIR), which can suppress proliferation of host hepatocytes, has been reported. We have conducted experiments that focused on less invasive and clinically applicable techniques and progenitor cells. First, DPP4-F344 rats underwent partial HIR and PVBL of one lobe, followed by intrasplenic hepatocyte transplantation at 1 × 10(7). Second, after partial HIR and PVBL, two types of progenitor cells were transplanted (small hepatocytes (SHs) or adipose-derived mesenchymal stem cells (ADSMC)).RESULTS:16 wk after transplantation, the donor cells constituted > 70% of the hepatocytes of the irradiated lobe, showing Cx32, PCK-1, and glycogen storage. The SHs grew more quickly than the hepatocytes. Although the ADSMCs were engrafted after 8 wk, their proliferation was not observed.

Free Research Field

再生医療学