New modality for cell-replacement therapy using adult liver-derived progenitor cells for post-pancreatectomy diabetic patients.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462108
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Shinshu University
• Principal Investigator: KOBAYASHI Akira 信州大学, 学術研究院医学系, 准教授 (90334903)
• Co-Investigator(Kenkyū-buntansha): MOTOYAMA Hiroaki 信州大学, 学術研究院医学系(医学部附属病院), 助教 (20569587) ; MIYAGAWA Shin-ichi 信州大学, 学術研究院医学系, 教授 (80229806)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 分化転換 / インスリン産生細胞 / 転写因子

Outline of Final Research Achievements

The transcription factor (TFs)-mediated transdifferentiation is novel process to convert one adult cell type into an alternate cell type with a different function. This study focuses on the inducibility of TFs-mediated transdifferentiation using portal branch ligation-stimulated hepatic cells, which is bipotent hepatic progenitor cell clones, by culturing the cells in a serum-free differentiation medium containing specific compounds (SFDM) and/or using a three-dimensional (3D) cellular aggregation technique. We revealed that both treatment with SFDM and the use of a 3D culture acted interdependently to promote TFs-mediated transdifferentiation. This study provides an important strategy for the cellular transdifferentiation of adult somatic cells using defined factors.

Free Research Field

膵臓外科学