Establishment of early diagnosis of enterohepatic Helicobacters infection for prophylaxis and treatment of biliary tract cancers

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462112
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Shimane University
• Principal Investigator: Tajima Yoshitsugu 島根大学, 医学部, 教授 (20264228)
• Co-Investigator(Kenkyū-buntansha): URANO Takeshi 島根大学, 医学部, 教授 (70293701) ; NISHI Takeshi 島根大学, 医学部, 特別協力研究員 (80538893)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 胆道発がん / 胆汁耐性ヘリコバクター属菌 / ビリス菌 / ヘパティクス菌 / モノクローナル抗体

Outline of Final Research Achievements

(1) We developed four or two monoclonal antibodies against Gram-negative enterohepatic Helicobacters, Helicobacter bilis or Helicobacter hepaticus, respectively. The Helicobacter hepaticus genome has been sequenced. For the epitope analysis the Helicobacter bilis genome was sequenced.
(2) We developed in vitro isolation and culture method of hamster bile duct epithelial cells. We performed a comprehensive analysis of upregulated or downregulated gene sets in these cells infected with Helicobacter bilis or Helicobacter hepaticus using DNA microarray.
(3) We developed two Helicobacter bilis or Helicobacter hepaticus infection models against hamster (5-week-old, female) and mouse (6-week-old, female).

Free Research Field

肝胆膵外科学