The development of new recognition system for cancer stem cell-like cell using RNA aptamer

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462113
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: National Cancer Center Japan (2015); Yamaguchi University (2013)
• Principal Investigator: YOSHIMURA KIYOSHI 国立研究開発法人国立がん研究センター, 先端医療開発センター, 分野長 (30346564)
• Co-Investigator(Kenkyū-buntansha): KUREAMASU ATSUO 山口大学, 医学(系)研究科(研究院), 准教授 (90302091)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 癌幹細胞 / 膵癌幹細胞 / 免疫療法 / ＲＮＡアプタマー

Outline of Final Research Achievements

Cancer stem cells (CSCs) have been studied of their self-renewal capacity and pluripotency, as well as their resistance to anti-cancer therapy and their ability to metastasize to distant organs. CSCs are difficult to study because their population is quite low in tumor specimens. To overcome this problem, we established a culture method to induce a cancer stem-like cell (CSLC)-enriched population from human cancer cell lines. Here is the problem. The surface marker of CSLC was not purely identified. CSLC is mostly recognized by combined surface markers. To develop the immunotherapy targeting CSLC, we attempted the novel detection system using RNA aptamer.

Free Research Field

腫瘍免疫学、消化器外科学、腫瘍外科学