Inhibition of nestin phosphorylation in pancreatic cancer cells

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462127
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology
• Principal Investigator: Matsuda Yoko 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 研究員 (20363187)
• Co-Investigator(Kenkyū-buntansha): Matsushita Akira 日本医科大学, 医学部, 助教 (70449263) ; Shinji Seiichi 日本医科大学, 医学部, 助教 (80409193) ; Ishiwata Toshiyuki 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 研究部長 (90203041)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 膵癌 / nestin / 幹細胞 / 細胞骨格 / リン酸化 / 浸潤 / 転移 / 増殖

Outline of Final Research Achievements

In this study, we examined the roles of phosphorylated nestin in pancreatic cancer cells. We found nestin phosphorylation in mitotic phase of human pancreatic cancer cells. Nestin phosphorylation was correlated with MIB-1 index in human pancreatic cancer tissues. Transfection of pancreatic cancer cells with nestin mutated at one phosphorylation site inhibited cell growth. Transfecting cells with wild-type nestin or with nestin mutated at one site increased migration and invasion. In addition, transfection with nestin mutated at two phosphorylation sites inhibited migration, invasion, and liver metastases. Nestin phosphorylation at these two sites was decreased upon treatment with inhibitors for cyclin dependent kinases, AKT, and Aurora. Inhibiting nestin phosphorylation at these two sites may represent a novel therapeutic strategy for pancreatic cancer.

Free Research Field

消化器病理