2016 Fiscal Year Final Research Report
The drug delivery application of the ischemic myocardium targeting peptide
Project/Area Number |
25462152
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Cardiovascular surgery
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Research Institution | Osaka Medical College |
Principal Investigator |
Kanki Sachiko 大阪医科大学, 医学部, 助教 (40411350)
|
Co-Investigator(Renkei-kenkyūsha) |
MIENO Shigetoshi 大阪医科大学, 医学部, 非常勤講師 (10411373)
|
Project Period (FY) |
2013-04-01 – 2017-03-31
|
Keywords | 虚血性心筋症 / ホーミングペプチド / ペプチド創薬 / 培養細胞 / プロテオーム解析 |
Outline of Final Research Achievements |
To identify the mechanism of the ischemic myocardium targeting peptide that was found by in vivo phage display in rats, we performed the pull down assay with the peptide -tagged nano magnetic beads in the rats' ischemic myocardium. We harvested 10 bands from a gel-electrophoresis and analyzed them with mass finger printing by mass-sectrometory combined with MALDI-TOF. We obtained 5 candidate proteins. In order to verify whether these proteins are receptors of the homing peptide, we constructed recombinant candidate proteins that are tagged with fluorescence. To establish in vitro ischemia-reperfusion condition with cells, we succeeded to use a chemical condition with sodium cyanide instead of with a hypoxic chamber. We confirmed the cell damage by LDH release and ATP generation by the cells. Although the chemical ischemia-reperfusion worked and mimic the in vivo ischemia-reperfusion, the cells did not absorb the fluorecence-tagged homing peptide.
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Free Research Field |
心臓血管外科
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