• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2015 Fiscal Year Final Research Report

Therapeutic angiogenesis with preconditioned stem cells for clinical settings

Research Project

  • PDF
Project/Area Number 25462162
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Cardiovascular surgery
Research InstitutionYamaguchi University

Principal Investigator

KATSURA Shunsaku  山口大学, 医学部附属病院, 助教 (40457304)

Co-Investigator(Kenkyū-buntansha) HAMANO Kimikazu  山口大学, 大学院医学系研究科, 教授 (60263787)
HOSOYAMA Tohru  山口大学, 大学院医学系研究科, 助教 (20638803)
LI Tao-sheng  長崎大学, 原爆後障害医療研究所, 教授 (50379997)
Project Period (FY) 2013-04-01 – 2016-03-31
Keywords細胞移植治療 / 末梢血単核球 / 低酸素プレコンディショニング / 前臨床試験
Outline of Final Research Achievements

Peripheral blood mononuclear cell (PBMNC) is one of powerful tools for therapeutic angiogenesis in hindlimb ischemia. However, traditional approaches with transplanted PBMNCs show poor therapeutic effects in severe ischemia patients. In this study, we used autograft models to determine whether hypoxic pretreatment effectively enhances the cellular functions of PBMNCs and improves hindlimb ischemia. Preconditioned rabbit PBMNCs showed significantly enhanced functional capacities in resistance to oxidative stress, and cell viability. In addition, autologous transplantation of preconditioned rabbit PBMNCs significantly induced new vessels and improved limb blood flow. Our study demonstrated that hypoxic preconditioning of PBMNCs is a feasible approach for increasing the retention of transplanted cells and enhancing therapeutic angiogenesis in ischemic tissue.

Free Research Field

小児外科

URL: 

Published: 2017-05-10  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi