2016 Fiscal Year Final Research Report
Improvement of damaged lung by type II alveolar epithelial cells with multipotency - for the purpose of clinical application of lung transplantation -
Project/Area Number |
25462172
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Respiratory surgery
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Research Institution | Chiba Cancer Center (Research Institute) (2016) Chiba University (2013, 2015) |
Principal Investigator |
Yoshida Shigetoshi 千葉県がんセンター(研究所), 呼吸器外科, 部長 (90334200)
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Research Collaborator |
WADA HIRONOBU 千葉大学, 医学部附属病院, 助教 (90514604)
HATA ATSUSHI 千葉大学, 医学部附属病院, 医員 (30750806)
SHIINA YUKI 千葉大学, 医学部附属病院, 医員
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Project Period (FY) |
2013-04-01 – 2017-03-31
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Keywords | 呼吸器外科学 / 移植 / 再生 |
Outline of Final Research Achievements |
As a preliminary experiment, to stabilize the supply of type II alveolar epithelial cells (AEC-II) transferring in the injury lungs, we experimented the induction of differentiation from mouse induced pluripotent stem cells (iPS cells) to AEC-II. In addition, we established the mouse chronic obstructive bronchiolitis (BO) model after allogeneic lung transplantation, and the exhaustive gene analysis of the BO lung was performed. To image the kinetics of transferred AEC-II, the luciferase-transfected A549 cells were intravenously administered to mice, we confirmed the engraftment, distribution and proliferation of the cells in the lung. We will be transferred ACE-II induced from iPS cells into mouse injury lung in next experiment, and the possibility of clinical application of cadaveric lung transplantation will be pursued.
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Free Research Field |
医歯薬学
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