The analysis of predictive markers for the efficacy of cisplatin and vinorelbine regimens as postoperative adjuvant chemotherapy of lung cancer

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462174
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory surgery
• Research Institution: Kyoto University
• Principal Investigator: SONOBE Makoto 京都大学, 医学(系)研究科(研究院), 准教授 (00432378)
• Co-Investigator(Kenkyū-buntansha): DATE Hiroshi 京都大学, 大学院医学研究科, 教授 (60252962)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 分子マーカー / 補助化学療法

Outline of Final Research Achievements

Vinorelbine(VNR)-resistant lung cancer cell line has been made by step-by-step dose escalation method. Whole transcriptome was compared between the parental and the VNR-resistant lines using cDNA microarray. The comparison showed the significant increase of expression of ABCB1 transporter gene and extracellular matrix-integrin-Src pathway genes. The activation of ABCB1, multi-drug resistant, transporter was confirmed to be one of the main acquired resistant mechanisms. Furthermore, the association of focal adhesion pathways with VNR-resistant mechanism was analyzed. Both mechanisms synergistically affected on VNR resistance of lung cancer cells, and their concomittant inhibiton overcame the resistance. Clinical significance of the molecular expression of both pathways did not seen in the adjuvant settings of resected lung cancer patients. Our results were presented in some international or domestic conferences, and then, now prepared for being published.

Free Research Field

呼吸器外科