2015 Fiscal Year Final Research Report
Novel biomarkers that assist in accurate discrimination of squamous cell carcinoma from adenocarcinoma of the lung
| Project/Area Number |
25462185
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Respiratory surgery
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Kenji 順天堂大学, 医学部, 教授 (10415523)
KOGO Yasushi 独立行政法人理化学研究所, オミックス基盤研究領域, 研究員 (20462682)
KAWAI Jun 独立行政法人理化学研究所, オミックス基盤研究領域, 研究員 (30391923)
Oh Shiaki 順天堂大学, 医学部, 准教授 (50306958)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 肺癌 / 腺癌 / 扁平上皮癌 / 鑑別マーカー / SPATS2 / ST6GALNAC1 |
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| Outline of Final Research Achievements |
Precisely diagnosing squamous cell carcinoma (SCC) and adenocarcinoma (AD) is important to select the most effective treatment regimen for lung cancer, but this has been challenging in cases with poorly differentiated SCC (PDSCC) and AD without a lepidic growth component (non-lepidic AD). We approached this problem with a genome-wide technology to monitor promoter activities, Cap Analysis Gene Expression (CAGE). Based on the genome-wide profiles of 97 frozen tissues from surgically resected lung cancers, we identified two novel markers, SPATS2 for SCC and ST6GALNAC1 for AD. The expression of known molecular markers used in immunohistochemical analysis (IHC) for SCC (CK5, CK6, p40 and desmoglein-3) and AD (TTF-1 and napsin A) were different between SCC and AD. We confirmed their performance with an IHC and also confirmed the findings in another group of 74 patients.
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| Free Research Field |
呼吸器外科
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