2015 Fiscal Year Final Research Report
Repairing mechanism of human mesenchymal stem cells (hMSCs) via immune regulation on ischemia brain injury
| Project/Area Number |
25462236
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurosurgery
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| Research Institution | Showa University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SHIODA SEIJI 星薬科大学, 先端生命科学研究所, 特任教授 (80102375)
Rakwal Randeep 筑波大学, 生命科学研究科, 教授 (70590850)
MIYAZAKI TAKURO 昭和大学, 医学部, 講師 (80398693)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 骨髄間葉系幹細胞 / 脳虚血 / マイクログリア / マイクロアレイ |
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| Outline of Final Research Achievements |
Human bone marrow derived mesenchymal stem cells (hMSCs) attract the attention of tissue repair during several diseases. The purpose of the preset study is to determine the mechanism of hMSCs on tissue repair. In particular, we focused on the relationship between hMSCs and macrophages and microglia. hMSCs suppressed inducible nitric oxide synthase (iNOS) and NO production from BV-2 microglia after interferon-γ stimulation in the cell number dependent fashion. Moreover, by the transcriptome analysis, hMSCs cultured with ischemic hippocampal homogenate increased 98 transcriptomes including chemokines. Further analysis was determined a chemokine, CCL2 from hMSCs was regulated by inflammatory cytokines induced by ischemic stress.
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| Free Research Field |
神経免疫学
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