Acceleration of peripheral nerve regeneration with safety by tissue-engineered nerve conduits combined with induced pluripotent stem cell transplantation

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462300
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Osaka City University
• Principal Investigator: Uemura Takuya 大阪市立大学, 大学院医学研究科, 病院講師 (10597321)
• Co-Investigator(Kenkyū-buntansha): NAKAMURA Hiroaki 大阪市立大学, 大学院医学研究科, 教授 (60227931) ; OKADA Mitsuhiro 大阪市立大学, 大学院医学研究科, 講師 (40309571) ; TAKAMATSU Kiyohito 大阪市立大学, 大学院医学研究科, 客員准教授 (30295688)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: iPS細胞 / 人工神経 / 末梢神経 / 再生医療

Outline of Final Research Achievements

We have developed tissue-engineered nerve conduits in combination with iPS cells transplantation for peripheral nerve regeneration. In the present study, we reconstructed peripheral nerve gaps in mice using the nerve conduits coated with iPS cell-derived neurospheres and evaluated the teratoma formation and localization of engrafted cells histologically. The iPS cell-derived Schwann-like cells survived in the nerve conduits, especially around the distal nerve stump, until at least 14 days after transplantation. This finding suggests that transplanted Schwann-like cells derived from iPS cells may contribute to axon regeneration at the distal side. No teratomas were identified in the nerve conduits at 24 and 48 weeks after implantation.

Free Research Field

整形外科