Mechanisms of analgesic action by granulocyte colony-stimulating factor in the spinal dorsal horn

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462303
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Wakayama Medical University
• Principal Investigator: Tsutsui Shunji 和歌山県立医科大学, 医学部, 講師 (70423960)
• Co-Investigator(Kenkyū-buntansha): Taniguchi Wataru 和歌山県立医科大学, 医学部, 助教 (20453194)
• Co-Investigator(Renkei-kenkyūsha): Sonekatsu Mayumi 和歌山県立医科大学, 医学部, 学内助教 (40725579) ; Nishio Naoko 和歌山県立医科大学, 医学部, 特別研究員 (40648359)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 顆粒球コロニー刺激因子 / G-CSF / パッチクランプ法 / in vivoパッチクランプ法 / 脊髄後角 / シグナル伝達 / 興奮性シナプス後電流 / 抑制性シナプス後電流

Outline of Final Research Achievements

We investigated effects of Granulocyte colony-stimulating factor (G-CSF) onto the synaptic transmission of the rat spinal dorsal horn neurons by patch-clamp methods. In our study, bath application of G-CSF tended to enhance spontaneous excitatory postsynaptic currents (sEPSCs) of dorsal horn neurons. On the other hand, G-CSF had no effect onto spontaneous inhibitory postsynaptic currents (sIPSCs) of dorsal horn neurons. Moreover, we analyzed how G-CSF affected dorsal horn neurons by in vivo patch-clamp. It showed that sEPSCs were slightly inhibited by bath application of G-CSF. These results suggest that dorsal horn neurons have cell-specific response to G-CSF. We think that inhibitory effects onto excitatory neurotransmission by G-CSF lead analgesic actions.

Free Research Field

整形外科学