Effect of amiloride on endoplasmic reticulum stress response in the injured spinal cord of rats

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462311
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Tokai University
• Principal Investigator: WATANABE Masahiko 東海大学, 医学部, 教授 (40220925)
• Co-Investigator(Kenkyū-buntansha): SUYAMA Kaori 東海大学, 医学部, 助教 (20433914)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 脊髄損傷 / 小胞体ストレス / オリゴデンドロサイト前駆細胞 / アミロライド

Outline of Final Research Achievements

After spinal cord injury, secondary injury results in an expanding area of glial cell apoptosis. Oligodendrocyte precursor cells (OPC), that are involved in remyelination, have been shown to actively proliferate, but a majority of these cells succumb to apoptosis instead of differentiating into functional oligodendrocytes. In this study, We focused on endoplasmic reticulum (ER) stress as a cause of apoptosis of oligodendrocyte precursor cell following spinal cord injury, and examined it in vivo. We labeled proliferating OPCs and demonstrated that amiloride treatment led to greater numbers of OPCs and also oligodendrocytes in the injured spinal cord. The increased MBP expression suggests that the increased numbers of mature oligodendrocytes led to improved remyelination, which significantly improved motor function recovery.

Free Research Field

整形外科学