2015 Fiscal Year Final Research Report
Detection of circulating tumor cells targeting tumor specific fusion gene in musculoskeletal sarcoma
| Project/Area Number |
25462325
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Niigata University |
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Principal Investigator |
Hotta Tetsuo 新潟大学, 医歯学総合病院, 准教授 (00272815)
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| Co-Investigator(Kenkyū-buntansha) |
KAWASHIMA Hiroyuki 新潟大学, 医歯学系, 講師 (30361900)
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| Research Collaborator |
SASAKI Taro
YAMAGISHI Tetsuro
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 血中循環微量腫瘍細胞 / 骨軟部肉腫 / 融合遺伝子 / 遺伝子増幅 |
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| Outline of Final Research Achievements |
We developed a new technique to detect the specific gene expression in musculoskeletal sarcoma cells. Highly purified RNA was extracted from the 2 mL blood sample contaminated with 5 sarcoma cells. The condition of gene amplification was optimized and the target gene could be sensitively detected. Furthermore, the target gene was also detected in the blood sample from a patient who carried lung metastasis of musculoskeletal sarcoma.
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| Free Research Field |
骨軟部腫瘍
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