Study for the effect of radiation therapy amplication by acridine orange photosensitizer

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462328
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Mie University
• Principal Investigator: MATSUBARA TAKAO 三重大学, 医学(系)研究科(研究院), 講師 (30422827)
• Co-Investigator(Kenkyū-buntansha): MATSUMINE AKIHIKO 三重大学, 医学系研究科, 准教授 (00335118)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 放射線耐性 / アクリジンオレンジ療法 / 低酸素耐性 / CA9

Outline of Final Research Achievements

Acridine orange （AO） which is a photosensitizer accumulated to human osteosarcoma, lung cancer and breast cancer cell lines under the tumor microenvironmental condition (acidic, low oxygen and high hydrostatic pressure). There was amplification effect of about 1.5-2 times by changing tumor microenvironment, and, it was confirmed to have a higher cytotoxic effect against tumors in such specific condition. In the study of AO therapy for registrant strains. AO have a good citocydal effect for registrant strains, but there was no amplication effect in combination with AO and the protein obtained gene expression due to the tumor-specific extracellular environment analysis. While we revealed that CA9 is one of good target for osteosarcoma therapy, and that CA9 inhibitor suppressed the invasiveness and metastatic potential. In future, combination with CA9 and anti-tumor therapy can control local tumor recurrence and metastatic potential for sarcoma patients.

Free Research Field

骨軟部腫瘍