2015 Fiscal Year Final Research Report
Development of new multiplex PCR assay for preiprosthetic joint infection
| Project/Area Number |
25462346
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Yokohama City University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
INABA Yutaka 横浜市立大学, 附属病院, 准教授 (40336574)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 人工関節周囲感染 / 診断 / PCR |
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| Outline of Final Research Achievements |
Molecular diagnostic techniques can contribute to the diagnosis of periprosthetic joint infection (PJI). We have developed a new multiplex real-time polymerase chain reaction (PCR) assay. The most prominant feature of our PCR assay is specific detection of methicillin-resistant staphylococcus (MRS) and distinguish between Gram-positive and -negative bacteria.
This novel multiplex real-time PCR system has several advantages compared to previously reported methods and has potential uses in the diagnosis of PJIs, particularly those caused by viable but non-culturable organisms.
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| Free Research Field |
整形外科学
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