Treatment of Bone Nonunion Using Vascularized Tissue-Engineered Bone Graft

2016 Fiscal Year Final Research Report

• Project/Area Number: 25462351
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Nara Medical University
• Principal Investigator: Murata Keiichi 奈良県立医科大学, 医学部, 研究員 (10382318)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 再生医療 / 間葉系幹細胞 / βTCP / 血管 / 骨新生 / 血管誘導

Outline of Final Research Achievements

We developed a novel vascularized tissue-engineered bone (VTEB). Using rat models, a vascular bundle of femoral artery and vein was passed through the groove of the β-tricalcium phosphate ceramics (βTCP). Osteogenic matrix cell sheets (OMCS) wrapped around vascular bundles within βTCP. Four weeks after the implantation of the vascular pedicle, the VTEB was elevated as a single vascular peddled VTEB, implanted to the same site wrapping with a silastic sheet to prevent blood supply from surrounding tissue. Additional 4 weeks after this manipulation, the VTEB was harvested to evaluate angiogenic and osteogenic potentials. As a results, the VTEB combined with OMCS maintained a high angiogenic and osteogenic potential even after elevation as a pedicled VTEB. From the results of this study, the vascularized βTCP combined with OMCS is expected to be a powerful tool of bone reconstruction for the treatment of intractable nonunions with massive bone defects.

Free Research Field

整形外科　運動器再建外科