Differential gene expression of mesenchymal stem cells derived from bone-marrow and synovial-tissues

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462362
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: EZURA Yoichi 東京医科歯科大学, 難治疾患研究所, 准教授 (50333456)
• Co-Investigator(Renkei-kenkyūsha): SEKIYA Ichiro 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (10345291)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 間葉系幹細胞 / 組織間質細胞 / 間葉系前駆細胞 / 軟骨細胞分化能

Outline of Final Research Achievements

Appropriate "quality check (QC)" of mesenchymal stem cells (MSC) for regenerative therapy requires practical and quantitative marker set. Here, to establish such marker set, we investigated the profiles of gene expression in human MSCs derived from bone-marrow and synovial-tissues. By clarifying that mouse stromal progenitor cells derived from bone-marrow and synovial-tisues also showed similar differential pattern of the gene expression to that observed in human MSCs, we explored the marker set that would predict the in vitro chondrogenic potentials of the MSCs and stromal progenitor cells. Also, the potential for the unwanted ectopic calcification was evaluated by in vitro culture assays and gene expression.

Free Research Field

骨代謝学