New approach of hyaluronan as osteoarthritis drug and analysis of articular cartilage protection mechanism

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462372
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Okayama University
• Principal Investigator: Ohtsuki Takashi 岡山大学, 保健学研究科, 非常勤研究員 (10534802)
• Co-Investigator(Kenkyū-buntansha): Hirohata Satoshi 岡山大学, 大学院保健学研究科, 教授 (90332791) ; Nishida Keiichiro 岡山大学, 大学院医歯薬学研究科, 準教授 (80284058)
• Co-Investigator(Renkei-kenkyūsha): Fujibuchi Wataru 京都大学, iPS研究所, 教授 (60273512) ; Shinohata Ryoko 岡山大学, 大学院保健学研究科, 助教 (70335587) ; Kusachi Shozo 岡山大学, 大学院保健学研究科, 特任教授 (30214943) ; Ninomiya Yoshifumi 岡山大学, 大学院医歯薬学研究科, 教授 (70126241)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 軟骨 / マトリックス / ADAMTS / 変形性関節症

Outline of Final Research Achievements

Detection of miRNAs control cytokine induced aggrecanase We detected several miRNA s using miRNA array technique.Long term hyaluronan (HA) treatment reduced cartilage destruction, significantly, but its protection was limited.We revealed mechanical stress did not change HA receptor genes CD44 and ICAM1 mRNA expression level in chondrosarcoma cell line OUMS-27.Weak mechanical stress induced cartilage matrix proteins, both aggerecan and type 2 collagen in OUMS-27.

Free Research Field

マトリックスバイオロジー