Involvement of endoplasmic reticulum stress induced by mechanical stress in the pathogenesis of cartilage degeneration

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462374
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Kumamoto University
• Principal Investigator: Hirose Jun 熊本大学, 医学部附属病院, 准教授 (40433007)
• Co-Investigator(Kenkyū-buntansha): MIZUTA HIROSI 熊本大学, 大学院生命科学研究部, 教授 (60174025) ; OKAMOTO NOBUKAZU 熊本大学, 大学院生命科学研究部, 助教 (70600162)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 軟骨変性 / 小胞体ストレス / 酸化ストレス / カルボニルストレス

Outline of Final Research Achievements

To clarify the activation and relationship of oxidative stress, carbonyl stress and endoplasmic reticulum stress in chondrocytes induced by mechanical stress against articular cartilage, we evaluated the role of the three intracellular stresses on chondrocyte apoptosis and cartilage degeneration. In cultured rat articular chondrocytes, hyper mechanical loading increased all three intracellular stresses. These reactions were involved with crosslink of each stress and chondrocyte dysfunction and apoptosis. In the experimental rat OA model caused by knee instability, treatment of the each stress suppressor reduced the corresponding stress and cartilage degeneration.

Free Research Field

医歯薬学