The mechanisms of arthritic diseases-specific cartilage destruction by MMP molecules

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462379
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Saitama Medical University
• Principal Investigator: Araki Yasuto 埼玉医科大学, 医学部, 講師 (10580839)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 関節リウマチ / 変形性関節症 / MMP / エピジェネティクス / ヒストン修飾

Outline of Final Research Achievements

To clarify dysfunctions of epigenetic regulation in the pathogenesis of rheumatiod arthritis (RA), gene expression of matrix metalloproteinases (MMPs), which are matrix-degrading enzymes, and histone modifications in the promoters were examined in RA synovial fibroblasts (SFs) and osteoarthritis (OA) SFs. Gene expression of MMP-1, 3, 9, and 13 was significantly higher in RASFs than in OASFs. Histone methylation profiles in the MMP promoters were consistent with the MMP gene expression. In addition, MMP-1, 3, and 13 genes were responsive to IL-6 stimulation in RASFs. Taken together, it is suggested that histone methylation plays an important role in the activation of RASFs.

Free Research Field

リウマチ・膠原病