Application of biliverdin/bilirubin redox cycle to the novel therapeutic strategy against acute lung injury

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462450
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology
• Research Institution: Okayama Prefectural University
• Principal Investigator: Toru Takahashi 岡山県立大学, 保健福祉学部, 教授 (40252952)
• Co-Investigator(Kenkyū-buntansha): MORIMATSU Hiroshi 岡山大学, 医歯薬学総合研究科, 教授 (30379797) ; OGINO Tetsuya 岡山県立大学, 保健福祉学部, 教授 (90252949) ; SHIMIZU Hiroko 岡山大学, 医学部, 客員研究員 (80423284)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 急性肺傷害 / 出血性ショック / ビリベルジン / 炎症 / 酸化ストレス / 酸化的DNA損傷 / 抗炎症作用 / 抗酸化ストレス作用

Outline of Final Research Achievements

Hemorrhagic shock and resuscitation (HSR) induces pulmonary inflammation that leads to acute lung injury. Biliverdin, a metabolite of heme catabolism, has been shown to have potent anti-inflammatory, and anti-oxidant effects. We administered biliverdin to a rat model of HSR and examined its effect on HSR-induced lung injury. We found that pre-treatment of HSR rats with biliverdin at a dose of 35 mg/kg markedly ameliorated HSR-induced pulmonary inflammation and oxidative tissue injury in the lung as judged by the histopathological improvement, and the decrease in inflammatory gene expression and DNA oxidative damage without abnormal increase in serum bilirubin levels. We also confirmed that biliverdin administration after HSR had protective effects on lung injury. Our findings suggest that biliverdin has a protective role, at least in part, against HSR-induced lung injury through anti-inflammatory and anti-oxidant mechanisms.

Free Research Field

麻酔・蘇生学