2015 Fiscal Year Final Research Report
| Project/Area Number |
25462453
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Anesthesiology
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| Research Institution | Tokai University |
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Principal Investigator |
AJIMI Junko 東海大学, 医学部, 助教 (80328111)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIKAWA Masanobu 東海大学, 医学部, 准教授 (90276791)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | dynorphin / peptidase / toxicity / cancer pain |
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| Outline of Final Research Achievements |
The purpose of the present study was to evaluate antinociceptive potential and toxicity with administration of Dyn under pretreatment with a mixture of amastatin (A), captopril (C), and phosphoramidon (P), and/or Dyn-converting enzyme inhibitor (p-hydroxymercuribenzoate; PHMB). MALDI-TOF-MS analysis identified Dyn A (1-6) and Dyn A (1-10) fragments as products following incubation of Dyn A (1-17) with membrane preparation of rat midbrain with a mixture of the three PIs. Pretreatment with a mixture of the three PIs produced an approximately 30-fold augmentation in antinociception induced by low-dose administration of Dyn A. Dyn-induced antinociception and toxicity under pretreatment with various combinations of the three PIs and PHMB was greater than that with a mixture of the three PIs alone. These findings suggest that administration of a mixture of the three PIs increases Dyn A-induced antinociception under physiological conditions without toxicity.
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| Free Research Field |
鎮痛
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