Involvement of GPR40 in the production of central post-stroke pain after global cerebral ischemia

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462458
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology
• Research Institution: Kobe Gakuin University
• Principal Investigator: Tokuyama Shogo 神戸学院大学, 薬学部, 教授 (70225358)
• Co-Investigator(Kenkyū-buntansha): NAKAMOTO Kazuo 神戸学院大学, 薬学部, 講師 (30432636) ; HARADA Shinichi 神戸学院大学, 薬学部, 講師 (60633443)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 脳卒中後疼痛 / 長鎖脂肪酸 / GPR40 / 全脳虚血ストレス

Outline of Final Research Achievements

Central post-stroke pain (CPSP), one of the complications of cerebral ischemia and neuropathic pain syndrome, is associated with specific somatosensory abnormalities.We recently reported that DHA and/or GW9508, a GPR40 agonist, have suppressive effects on pain behavior elicited by formalin, acetic acid and a complete Freund's adjuvant. These effects appear to be mediated through the release of β-endorphin, an endogenous opioid peptide, which is stimulated by GPR40 signaling in the supraspinal region. The aim of the present study was to determine the involvement of GPR40 in the development of CPSP in an animal model of global cerebral ischemia. BCAO-induced mechanical hyperalgesia was significantly decreased by intracerebroventricular injection of docosahexaenoic acid or GW9508, a GPR40 agonist; furthermore, these effects were reversed by GW1100, a GPR40 antagonist.Our data show that BCAO-induced mechanical hyperalgesia can be regulated by stimulation of GPR40 signaling.

Free Research Field

精神神経薬理