Interaction between cancer stem cell from urothelial carcinoma and stromal cell in survival, proliferation, differentiation and migration.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462486
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Saga University
• Principal Investigator: yuji tokuda 佐賀大学, 医学部, 客員研究員 (90315200)
• Co-Investigator(Kenkyū-buntansha): TODA SHUJI 佐賀大学, 医学部, 教授 (80188755) ; NANRI MAKI 佐賀大学, 医学部, 助教 (00448474)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 膀胱癌 / 癌幹細胞 / 間質細胞

Outline of Final Research Achievements

Superficial bladder cancer might promote myofibroblast differentiation of adipose tissue stromal cells as a cancer-associate phenotype, whereas invasive bladder cancer might promote their adipocyte differentiation.We examined the expression profile of proteins in these types cultured alone or with ATSCs. The expression of CD147, galectin-3 and galectin-3BP increased in co-culture of superficial type and ATSCs, while that of CD147, galectin-3 and galectin-3BP decreased in co-culture of invasive type and ATSCs. The expression of cathepsin D and CRELD2 decreased in in co-culture of superficial type and ATSCs, while that of cathepsin D and CRELD2 increased in co-culture of invasive type and ATSCs. Opposite expression of CD147, galectin-3, galectin-3BP, cathepsin D and CRELD2 was observed in ATSC-affected superficial and invasive type. Thus, these molecules may be involved in the mechanisms of ATSC-affected differences between superficial and invasive type.

Free Research Field

泌尿器科