2015 Fiscal Year Final Research Report
The role of HMGB1 and RAGE in urinary stone formation
| Project/Area Number |
25462529
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Kanazawa Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MORITA Nobuyo 金沢医科大学, 医学部, 助教 (20440505)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | urolithiasis / calcium oxalate / HMGB1 / RAGE / MCP-1 |
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| Outline of Final Research Achievements |
We evaluate the relationship between urolithiasis and High Mobility Group Box1 (HMGB1) and Receptor of Advanced of Glycation Endoproduct (RAGE).
Renal epithelial HK-2 and MDCK cells were obtained and maintained. Calcium oxalate monohydrate (COM) crystals were suspended in serum-free medium at a final concentration of 500μg/ml. The total protein was extracted by phenol/ammonium acetate in methanol method, and total RNA was extracted by the acid guanidium thiocyanate-phenol-chloroform method. Western blot study revealed the expression of HMGB1. Expression of HMGB1 and monocyte chemoattractant protein-1 (MCP-1), which is induced by interaction between renal epithelial cells and COM crystals was detected by reverse transcription-polymerase chain reaction (RT-PCR). MCP-1 genes were also up-regulated with exposure of COM crystal.
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| Free Research Field |
尿路結石症
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