2015 Fiscal Year Final Research Report
Effect of anti-CD25 monoclonal antibody on chronic otitis media in mice model
| Project/Area Number |
25462648
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Oita University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI MASASHI 大分大学, 医学部, 教授 (60211314)
KAWANO TOSHIAKI 大分大学, 医学部, 助教 (30633424)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 慢性中耳炎 / インフルエンザ菌 / 制御性T細胞 / CD25 |
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| Outline of Final Research Achievements |
Nontypeable Haemophilus influenzae (NTHi) is associated with chronic otitis media (COM). In this study, we generated a murine model of COM by using Eustachian tube obstruction and NTHi inoculation into the tympanic bulla, and we investigated the relationship between regulatory T (T reg) cells and chronic inflammation in the middle ear. Middle ear effusions (MEEs) and middle ear mucosae (MEM) were collected at days 3 and 14 and at 1 and 2 months after inoculation. CD4+ CD25+ FoxP3+ T reg cells accumulated in the middle ear, and the percentage of T reg cells in the MEM increased for up to 2 months after inoculation. T reg cell depletion induced a 99.9% reduction of bacteria counts in MEEs and also significantly reduced the ratio of NTHi culture-positive MEE. The levels of these cytokines were also reduced in MEEs. In summary, we developed a murine model of COM and our findings indicate that T reg cells confer infectious tolerance to NTHi in the middle ear.
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| Free Research Field |
医歯薬学
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