Research for Elucidating Pathophysiological Mechanism in the Vocal Folds in Mice

2016 Fiscal Year Final Research Report

• Project/Area Number: 25462697
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Kyoto University (2016); The Tazuke Kofukai (2013-2015)
• Principal Investigator: Masaru Yamashita 京都大学, 医学研究科, 助教 (10635519)
• Co-Investigator(Renkei-kenkyūsha): MAETANI Toshiki 公益財団法人田附興風会医学研究所北野病院, 第5研究部, 研究員 (90346669) ; KANAI Rie 公益財団法人田附興風会医学研究所北野病院, 第5研究部, 研究員 (30574008) ; TSUJI Takuya 公益財団法人田附興風会医学研究所北野病院, 第5研究部, 研究員 (00543266)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: マウス声帯 / 内視鏡下手術 / 骨髄内骨髄移植

Outline of Final Research Achievements

The vocal folds have unique structure, which contains a "Pudding" layer on an intralaryngeal muscle layer. Many researchers had tried to elucidate pathophysiological mechanism in the vocal folds, but wound healing process and anatomical understanding are still unclear. We, therefore, established two new mouse surgery models. The first model is intra-bone bone marrow transplantation model in mice. To track bone marrow derived cells in the vocal folds, bone marrow tissue from transgenic mice with expressing green fluorescent protein was transplanted into femoral bones of wild-type mice. The other is an endoscopic injury model to vocal folds in mice. Since their vocal structures are very tiny, this procedure was very challenging without open cervical surgeries. We established new surgical tools to create vocal fold injuries without any lethal damage. The application of these models and commercially available gene-manipulated mice would enhance the researches in the vocal folds.

Free Research Field

喉頭科学