Control of ganglion cells for producing the On- and Off response using optogenetic tools.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462747
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Iwate University
• Principal Investigator: SUGANO ERIKO 岩手大学, 工学部, 准教授 (70375210)
• Co-Investigator(Kenkyū-buntansha): ISAGO Hitomi 東北大学, 大学病院, 助手 (30400451)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: オプトジェネティクス / 再生医学 / 生理学 / 脳・神経

Outline of Final Research Achievements

Modifierd vorvox derived channelrhodopsin-1 (mVChR1) or halorhodopisin(NpHR) gene was inserted into adeno-associated virus vector (AAV), and capsid modified AAV2 was most efficient vector for retinal ganglion cells. However, NpHR was low expression in mammalian cells by AAV gene transfer. We modified the transmembrane signal of NpHR {m(em)-NpHR}, however, there was not sufficient to improve the expression and function compared to that of ChR2 protein in vivo and vitro. We also studied about the 3-dimentional structural analysis of protein on data base, these analyze indicated that there might be a problem in the pathway of the ion and result in low light sensitivity.

Free Research Field

眼科学