Patch tracheoplasty in body tissue engineering using collagenous connective tissue membranes(biosheets)

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462780
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatric surgery
• Research Institution: Saitama Medical University
• Principal Investigator: Satake Ryosuke 埼玉医科大学, 医学部, 講師 (70597525)
• Co-Investigator(Kenkyū-buntansha): KOMURA Makoto 東京大学, 医学部付属病院, 特任研究員 (10422289) ; HOSHI Kazuto 東京大学, 医学部, 准教授 (30344451) ; TABATA Yasuhiko 京都大学, 再生医科学研究所, 教授 (50211371)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: biosheet / biotube / 気管再生 / 軟骨再生

Outline of Final Research Achievements

【Background】The aim was to evaluate the use of biosheets(collagenous connective tissue membranes are useful for engineering cardiovascular tissue) as a potential substitute material on vivo in a rabit model.【Methods】Rectangular-shaped Gore-Tex was(Gruop 1) ,dermis(Group 2) and biosheets(Group3) implanted into the defect created in the midventral portion of the cervical trachea. Biosheets were prepared by embedding silicone moulds in dorsal subcutaneous pouches in rabbits.【Main Results】All materials maintained airway structure for up to 4 weeks after implantation. Regenetive cartilage in implanted biosheets in group 3 was confirmed by histological analysis. Tracheal epithelial regeneration occurred in the internal lumen of group 3. There were significant differences in the amounts of collagen type Ⅱ and glycosaminoglycan between group 3 and group 1 or 2.【Conclusion】We confirm that cartilage can self-regenerate onto an airway patch using biosheets.

Free Research Field

小児内視鏡外科