The quantitative evaluation of relationship between the axonal regeneration of the transplanted nerve and the blood flow volume of the nerve and the grafted site.

2016 Fiscal Year Final Research Report

• Project/Area Number: 25462787
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plastic surgery
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: TANAKA Kentaro 東京医科歯科大学, 医学部附属病院, 特任助教 (20569503)
• Co-Investigator(Renkei-kenkyūsha): MIURA Masahiko 東京医科歯科大学, 医歯学総合研究科, 教授 (10272600) ; WAKABAYASHI Yoshiaki 東京医科歯科大学, 医歯学総合研究科, 講師 (00431916) ; OU Gi 東京医科歯科大学, 生体材料工学研究所, 助教 (60451944) ; ICHINOSE Shizuko 東京医科歯科大学, 医歯学研究支援センター, 助教 (60014156)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 神経移植 / 移植神経の血流量 / 移植床の血流量 / 軸索再生 / 放射線照射 / 組織エタノール注入 / 神経再生の評価 / 再建手術

Outline of Final Research Achievements

The sciatic nerve graft was harvested as a vascularized nerve graft in rat model. Low blood flow scar tissue will be developed in the nerve-grafted area by an exposure to radiation in this experimental design, and it is the greatest characteristic of this study. But the irradiation device in our institution broke down and became non-usable. We were forced to make a significant change in our experimental plan, and tried to cause a scar tissue formation by injecting 100% ethanol into the nerve-grafted area as an alternate method. The nerve grafting experiments were performed in these rat models, and the axonal regeneration of the transplanted nerve was evaluated electrophysiologically. But the injected 100% ethanol diffused from the injected site after operation, and produced permanent damage not only to the grafted site but also to the transplanted nerve itself. Therefore we came to the conclusion that the alternative experiment was unsuitable for making an adequate conditions.

Free Research Field

形成外科学