Novel combination-oriented molecular-targeting therapy for keloid

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462798
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plastic surgery
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Nishino Kenichi 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (00138471)
• Co-Investigator(Kenkyū-buntansha): 酒井 敏行 京都府立医科大学, 医学(系)研究科(研究院), 教授 (20186993)
• Co-Investigator(Renkei-kenkyūsha): SAKAI Toshiyuki 京都府立医科大学, 医学研究科, 教授 (20186993) ; KAWARAZAKI Ayako 京都府立医科大学, 医学部附属病院, 専攻医 (50550498)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ケロイド / 線維芽細胞 / コラーゲン / MEK阻害剤 / HDAC阻害剤

Outline of Final Research Achievements

Keloids are fibro-proliferative diseases characterized by the accumulation of an extracellular matrix such as collagen. The aberrant proliferation and production of extracellular matrix have been related to the expression pattern of different cytokines, including TGF-β and IL-6 in keloid fibroblasts. The therapies which target to these cytokines and related molecules have possibility to ameliorate keloid. In this study, we evaluated the effects of HDAC inhibitor and MEK inhibitor, as the candidate chemicals for targeting the pathway, against keloid fibroblasts. HDAC inhibitor suppressed proliferation and production of collagen in keloid fibroblasts, and MEK inhibitor weakly suppresses those. Moreover, we investigated the effect of the combination of HDAC inhibitor and MEK inhibitor. As the results, the combination did not show the significant combined effect to suppress keloid fibroblasts.

Free Research Field

ケロイド