The examination of brief electrical stimulation for axonal regeneration and its application for diabetes mice

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462803
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plastic surgery
• Research Institution: Juntendo University
• Principal Investigator: Yuhei Natori 順天堂大学, 医学部, 非常勤助手 (80445478)
• Co-Investigator(Kenkyū-buntansha): MIZUNO Hiroshi 順天堂大学, 医学部, 教授 (80343606) ; AYATO Hayashi 順天堂大学, 医学部, 先任准教授 (10365645)
• Research Collaborator: YOSHIZAWA Hidekazu 順天堂大学, 医学部, 助手 (10512593) ; SENDA Daiki 順天堂大学, 大学院生
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: Thy1 YFP-16マウス / 短期的電気刺激 / 神経再生

Outline of Final Research Achievements

We studied nerve regeneration using Thy1 YFP-16 mice. There was correlation between fluorescence intensity and histological axon regeneration in Thy1 YFP-16 mice, with the number of regenerated axons showing the strongest correlation. In brief electrical stimulation, the speed of axon growth in early stage was faster in the 60-minute stimulation group as compared with other groups, suggesting that it may primarily reduce the period where regenerated axon travels beyond the crush site. Moreover, regeneration promoting effect was observed in motor nerves as well as sensory nerves, thus application to various scenes can be expected in the future.

Free Research Field

末梢神経