Analysis of genetic polymorphisms and gene expression profiles of post-cardiac arrest syndrome as predictive indicators for prognosis

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462813
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Emergency medicine
• Research Institution: Chiba University
• Principal Investigator: Eizo Watanabe 千葉大学, 医学(系)研究科(研究院), 准教授 (40375639)
• Co-Investigator(Kenkyū-buntansha): ODA Shigeto 千葉大学, 大学院医学研究院, 教授 (90204205)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 心肺停止 / 心停止後症候群 / 予後予測 / 敗血症 / 遺伝子多型 / 一塩基多型 / 遺伝子発現プロファイル

Outline of Final Research Achievements

The aim of the present study was to investigate association between outcome and genetic factor in the pathophysiology of post-cardiac arrest syndrome (PCAS), which is recognized as “sepsis-like syndrome”. Therefore, we performed gene expression profiling analyses for acute phase of the PCAS and compare them with respect to neurologic outcome of the patients. As the results, 90 transcripts were extracted with 14400 oligo DNA microarray by classifying according to neurologic outcome, i.e., cerebral performance category (CPC). Moreover, we identified 4 neutrophil function-related transcripts which altered over time (from ICU day 1 to day 5) according to 90 day outcome. We further analyzed inflammation-related gene polymorphisms using a single nucleotide polymorphism (SNP) chip which we designed, and found several candidate SNPs which were associated with outcome of critically ill patients including PCAS.

Free Research Field

救急集中治療医学