Development of new treatment using autologous artificial skin graft in patients with severe burn injury

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462823
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Emergency medicine
• Research Institution: Saga University
• Principal Investigator: Koami Hiroyuki 佐賀大学, 医学部, 助教 (10465354)
• Co-Investigator(Kenkyū-buntansha): SAKAMOTO YUICHIRO 佐賀大学, 医学部, 教授 (20366678)
• Co-Investigator(Renkei-kenkyūsha): NOGUCHI RYO 佐賀大学, 医学部, 助教 (70530187)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 3次元皮膚培養 / スフェロイド / 繊維芽細胞 / ケラチノサイト / 血管内皮細胞 / 熱傷モデル / ラット / 脂肪由来間葉系幹細胞

Outline of Final Research Achievements

We performed in vivo study in order to produce the artificial skin graft which possessed sufficient firmness to skin grafting. At first, we performed basic cell cultures and produced spheroids using fibroblasts, endothelial cells and keratinocyte, respectively. Second, we collect a lot of spheroids in one plate. Finally, gathering spheroids are transformed into one skin patch after one day. We could produce a skin patch including rich fibroblast, but couldn't form the patch by poor fibroblast or no fibroblast. Next, we utilize the fat-derived mesenchymal stem cells to accelerate stronger crosstalk between keratinocytes and fibroblasts. The new patch of keratinocyte including fat-derived mesenchymal stem cells is stronger and large. We will proceed basic experiments and assess the interactions of mesenchymal stem cells between keratinocytes and fibroblasts in the future.

Free Research Field

救急医学